Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration.
Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins.
Phosphorylation of paxillin occurs at both tyrosine and serine sites.
Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins.
Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin.
The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 detected Paxillin on 10ug of Pervandate treated and untreated Huvec cell lysate
Optimal dilutions to be determined by the researcher.
Positive Control:Pervandate treated and untreated Huvec cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS (no Ca2+, no Mg2+), pH 7.3, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human phospho-Paxillin (Ser126). Species crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide corresponding to human Paxillin at phosphorylated Ser126. Species Sequence homology: rat.
