84-1129-91　［取扱停止］MYPT1, phosphorylated (Thr696) (Myosin Phosphatase 1) 200ug　M9925-09A

特徴

• Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase.
• Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase.
• It is also involved in smooth muscle contraction.
• Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction.
• This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle.
• The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1.
• PP1c interacts with at least four binding sites on the amino-terminus of MYPT1.
• MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1ug/ml detects MYPT1 phosphorylated at Thr969 on 10ug of Calyculin/Okadaic treated and untreated HEK293 cell lysate.
• Optimal dilutions to be determined by the researcher.
• Positive Control:Calyculin/Okadaic treated and untreated HEK293 cell lysates.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.