84-1129-90　ISL1 (IISL LIM Homeobox 1, Insulin Gene Enhancer Protein ISL-1, Isl-1, ISLET1, Islet-1) 100ug　I8782-60A

特徴

• Islet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans.
• It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
• In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation.
• More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages.
• This gene encodes a member of the LIM/homeodomain family of transcription factors.
• The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression.
• The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation.
• Mutations in this gene have been associated with maturity-onset diabetes of the young.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5ug/ml detected ISL-1 on 10ug K562 cell lysate
• Immunohistochemistry (Paraffin): 1:300 dilution from a previous lot detected ISL-1 in mouse pancreas tissue.
• Antigen retrieval with citrate, pH 6.0 is recommeneded
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.