84-1129-83　TTK (TTK Protein Kinase, Dual Specificity Protein Kinase TTK, ECSTY Kinase, ESK, FLJ38280, hMPS1, Monopolar Spindle 1 Like 1, MPS1L1, Mps1, Phosphotyrosine Picked Threonine Kinase, PYT) 100ug　T9142-01E

特徴

• TTK (Dual specificity Thr/Tyr kinase; also PYT and hMps1) is a 97-105kD member of the Ser/Thr protein kinase family of enzymes.
• It is expressed in rapidly proliferating tissues and cell lines, and participates in cell cycle regulation.
• In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation.
• Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2.
• Human TTK is 857 (aa) in length and contains one protein kinase domain (aa525-791).
• Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686.
• Apoplications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile 40-50% glycerol, PBS.
• Aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.