TLE1 (Transducin-Like Enhancer of Split 1; also ESG1 and GRG1) is a member of the WD repeat Groucho/TLE family of transcriptional repressors.
It is widely expressed, and is known to antagonize NFkB and TCF (Wnt)-mediated signaling.
TLE1 binds to other family members as a heterooligomer, or to itself as a homooligomer.
While it possesses no intrinsic DNA-binding activity, it does modulate the activity of bound transcription factors.
Human TLE1 is 770 amino acids (aa) in length.
It contains a Gln-rich region that mediates oligomerization (aa1-131), a CCN domain that contains an NLS and CDC2 kinase site (aa200-268), and six WD repeats that mediate protein-protein interaction (aa470-767).
Phosphorylation on Ser will generate 90-93kD and 116kD forms in SDS-Page.
Potential splice variants exist.
There are alternative start sites at Met57 and Met326, a 10aa insertion after Gly124, and three distinct eight aa substitutions for aa 193-770, 193-199 and 445-770.
Over aa11-200, human TLE1 shares 98% aa identity with mouse TLE1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-1ug/ml.
The detection limit for rhTLE1 is approximately 2ng/well.
Western Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized power in PBS, 5% Trehalose. Reconstitute with sterile 40-50% glycerol, PBS.
Specificity：Recognizes human TLE1. In direct ELISAs, this antibody shows ~10% cross-reactivity with rhTLE3 and rhTLE4 and less than 5% cross-reactivity with rhTLE2.
