84-1129-79　SH2 domain protein 2A (SH2D2A, F2771, SCAP, SH2 domain-containing adapter protein, SH2 domain-containing protein 2A, T cell-specific adapter protein, TSAd, TSAD, VEGF receptor-associated protein, VRAP) 100ug　S1010-90K

特徴

• SH2D2A (SH2 domain-containing protein 2A; also TSAd and VRAP) is a 52kD protein best described as a multifunctional intracellular adaptor molecule.
• It is expressed in activated CD4+ and CD8+ T cells, endothelial cells, and fibroblasts.
• SH2D2A is found in both cytoplasm and nucleus, and has multiple binding partners.
• After TCR activation, SH2D2A interacts with LCK, prolonging its activity.
• It also binds to SMAD2 and SMAD3, and constitutively interacts with PI3K and PLCg, thus participating in VEGF:KDR signaling.
• Human SH2D2A is 389 amino acids (aa) in length and contains one SH2 domain (aa 95-186) and a Pro-rich region (aa 187-389).
• Phosphorylation occurs on Y39, Y216 and Y305, and on S217 and S296.
• Apoplications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile 40-50% glycerol, PBS.
• Aliquot and store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.