Human Relaxin-1/H1 is a 6kD member of the insulin/Relaxin gene superfamily.
Relaxin-1 is synthesized as a 185 amino acid (aa) preprohormone that is proteolytically processed into a nonglycosylated, disulfide-linked, 53 aa mature heterodimer that contains a 29 aa B chain (aa 25-53) and a 24 aa A chain (aa 161-185).
An alternate splice form of human Relaxin-2 is known that contains the B chain and an uncleaved 47 aa extension.
Mature human Relaxin-1 heterodimer is 48%, 44% and 43% aa identical to rat, dog and pig Relaxin-2, respectively.
Applications:Suitable for use in Western Blot and Direct ELISA.
Other applications not tested.
Recommended Dilutions:ELISA (direct): 0.5-1ug/ml.
Western Blot: 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized power in PBS, 5% Trehalose. Reconstitute with sterile 40-50% glycerol, PBS.
Specificity：Recognize human Relaxin-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows less than 5% cross-reactivity with rhRelaxin-2 and rhRelaxin-3.
