84-1129-77　Progesterone Receptor B, phosphorylated (S294) (NR3C3, PR-B) 100ug　P9007-31J

特徴

• Progesterone Receptor B (PR-B) is a 118kD member of the NR3 subfamily within the nuclear hormone receptor family of proteins.
• It is expressed in female reproductive tissues as well as neurons throughout the CNS.
• PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone.
• Human PR-B is 933 amino acids (aa) in length.
• It contains an N-terminal regulatory region aa 1-566), a DNA binding domain (aa 567-639), and a steroid-binding region aa 681-933).
• Ligand binding induces a key phosphorylation event at Ser294 catalyzed by ERK1/2, regulating cell growth, cell adhesion, and gene transcription.
• An alternate start site at Met165 generates 90kD PR-A, an isoform particularly important in the ovary and uterus that insures fertility.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute by adding sterile buffer.
• Aliquot and store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.