Palladin (a cytoarchitectural protein named after the 16 century Venetian architect Palladio) is a cytoplasmic member of the myotilin/palladin family of proteins.
Palladin is widely expressed and interacts with actin-associated proteins, thus promoting actin bundle stability.
Human Palladin is 1383 amino acids (aa) in length and contains five I-type Ig-like domains (aa 271-360; 440-539; 1001-1085; 1135-1226; 1233-1324), a Pro-rich region (aa 634-864) and nine potential Ser/Thr phosphorylation sites.
Multiple isoform variants exist.
Predominant are a 140kD and 92-95kD short form that show alternative start sites at Met383 and Met712, respectively.
The 140kD isoform also shows a deletion of aa 656-879 to generate an approximately 115kD isoform.
Apoplications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile 40-50% glycerol, PBS.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a lyophilized power in PBS, 5% Trehalose. Reconstitute with sterile 40-50% glycerol, PBS, 0.02% sodium azide.
Specificity：Recognizes human Palladin isoforms at 95, 115 and 140kD using Western blots.
