NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA) is a 17-20kD member of the NDK family of enzymes.
NM23-H1 is ubiquitous in expression, and performs multiple functions.
It forms disulfide-linked homohexamers, and heterohexamers with NM23-H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate.
It also shows histidine protein and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP.
It is found both intracellularly, and in blood at ng/ml concentrations.
Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5-134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125.
Human NM23-H1 shares 89% aa identity with human 17-18kD NM23-H2, and 94% aa identity with mouse NM23-H1.
A second H1 isoform named NM23-H1B with 25 additional aa at the N-terminus has also been described.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute by adding sterile buffer.
Aliquot and store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute in 100ul of PBS, 0.02% sodium azide.
Specificity：Recognizes human NM23-H1 (H2). Species Crossreactivity: mouse and rat.
