Interleukin-12 is produced by activated B lymphocytes and macrophages.
The properties of IL-12 include the stimulation of growth of activated T lymphocytes and the development of TH1 CD4 positive cells and CD8 positive cytotoxic T cells.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
The detection limit is ~50ng/lane under non-reducing and reducing conditions.
Neutralization: The ND50 was determined to be ~0.1-0.5ug/ml in the presence of 25ng/ml of recombinant canine IL-12, using PHA activated human peripheral blood mononuclear cells (PBMCs)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：canine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug (LAL)
Form：Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes canine IL-12. In direct ELISAs, ~40% crossreactivity with recombinant human IL‑12 p35/p40 and recombinant feline IL-12 p35/p40 is observed, and <10% crossreactivity with recombinant rat IL-12 p35/p40 is observed.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Ca
Immunogen：CHO-derived recombinant protein corresponding to canine interleukin 12 p40 (Ile23-Ser329) and p35 (Arg26-Ser222) (Asp63Glu, Tyr64Leu, Ile65Tyr, Pro66Ser), UniProt: Q28268 and Q28267, respectively.
