Tumor Necrosis Factor alpha (TNF-alpha) is a 17.4kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but it is also produced by T and B lymphocytes, activated monocytes and fibroblasts.
TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-?B, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions.
Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.
Applications:Suitable for use in ELISA, Immunohistochemistry, Functional Assays and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.25-1ug/mlWestern Blot: 0.1-0.2ug/mlImmunohistochemistry (paraffin): 2.5-5ug/ml Requires antigen retrieval using heat treatment prior to staining paraffin sections.
Use sodium citrate buffer, pH 6.0
Functional assays: 0.012-0.014ug/mlOptimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control:Human Breast Carcinoma
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS. No preservative added. Reconstitute with 100ul ddH2O.
