STAT3 is an 80-85kD member of the STAT protein family, plays an important role in cell growth, survival differentiation and transformation, transducing signals from cytokine receptors to the nucleus.
STAT3 is phosphorylated in response to cytokines and growth factors including interleukin-5, interleukin-6, epidermal growth factor and leukemia inhibitory factor.
It is expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Applications:Suitable for use in ELISA, Immunohistochemistry (frozen), and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (frozen): 10ug/mlELISA: 1:32,000Western Blot: 0-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in tris-saline, pH 7.3, 0.02% sodium azide, 0.5%BSA.
Specificity：Recognizes an epitope within the C-terminal (CT) region of human STAT3. Species Crossreactivity: mouse. Species sequence homology: rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Peptide sequence DMELTSECARSPM corresponding to the C-terminal region of STAT3 (NP_003141.2; NP_644805.1)
