Salmonella is a genus of the family Enterobacteriaceae populated by a variety of Gram negative rod-shaped bacteria, many of which are pathogenic and cause a range of diseases in humans.
Salmonellae possess 3 major surface antigens: the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae).
Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria.
This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:200-1:1,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 1% BSA, 0.002% thimerosal. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes Salmonella "O" and "H" antigens. Antiserum is unabsorbed and may crossreact with related Enterobacteriaceae.
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：Mixture of S. enteriditis, S. typhimurium and S. heidelburg.
