RIG-I is a receptor found in the cytoplasm that recognizes viral RNA from replicating viruses in infected cells.
Upon activation with intracellular RNA, a cascade is triggered resulting in the activation of NF-kappa-B, and the eventual induction of antiviral cytokines such as IFN-beta and RANTES.
RIG-I is essential for the production of interferons in response to RNA viruses and as such play a key role in innate immunity.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (paraffin): 5ug/ml detects a band at ~84kD in C2C12 cell lysate.
Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate, pH 6, is recommended
Western Blot: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control: Human heart
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography from whole serum.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human RIG-I. Species Crossreactivity: mouse and rat
