84-1129-19　［取扱停止］RAD9 (DNA repair protein RAD9, YDR217C) 50ug　R0444-68A

特徴

• Rad9 is a protein involved in the DNA damage checkpoint.
• In S.
• cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage.
• Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2.
• Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair.
• Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:2,000-1:4,000Western Blot: 1:50-1:250Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.