Programmed cell death 4 (Pdcd4) is a novel tumor suppressor.
Pdcd4 interacts with the RNA helicase eIF4A and the scaffolding protein eIF4G, two components of the translation initiation complex, and thus inhibits protein synthesis.
Pdcd4 has also been shown to inhibit transcription factor activator protein-1 (AP-1), which regulates several events required for cell invasion, including expression of matrix metalloproteinases and cell motility
Pdcd4 localizes to the nucleus in proliferating cells, and its expression is modulated by cytokines in natural killer and T cells.
Pdcd4 expression is down regulated with tumor progression in carcinomas of the lung, colon, prostate and breast.
Applications:Suitable for use in ELISA, Immunohistochemistry (paraffin), and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:100-1:300ELISA: 1:10,000-1:50,000Western Blot: 1:1,000-1:10,000Requires protein digestion pre-treatment of paraffin e.g.
trypsin or pronase
Optimal dilutions to be determined by the researcher.
Positive Control Tissue:Mouse colon
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity：Recognizes a specific epitope within the carboxy-terminal of human programmed cell death 4. Species sequence homology: rat, mouse, xenopus.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids near the carboxy terminal of human Pdcd4.
