The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac.
They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding.
Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding.
The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility.
Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding.
Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3.
This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment.
Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-1ug/mlImmunofluorescence: 20ug/mlImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat Cell Lysate or Mouse Spleen Tissue Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human PAK2. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：14 amino acid peptide sequence near the carboxy terminus of human PAK2.
