p65 (RelA) subunit of nuclear factor kappa-B (NFkB) when phosphorylated at serine 536.
NFkB is a heterodimeric DNA binding protein which was originally identified consisting of p65 and p50 (NFkB1) subunits.
Research has led to the discovery of other subunits, including p52 (NFkB2), cRel, and RelB.
NFkB is ubiquitously expressed, and functions as a second messenger which upon activation leads to the transcription of a number of genes.
NFkB is activated by a number of agents, including pro-inflammatory cytokines and bacterial lipopolysaccharide, and is essential for the maturation of T cells and regulation of their survival and activation.
Phosphorylation of serine 536 contributes to NFkB transactivation and increases p65 transcription activity.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (paraffin): 1:50-1:250ELISA: 1:500-1:3,000Western Blot: 1:100-1:1,000Optimal dilutions to be determined by the researcher.
Histology Positive Control Tissue:Human kidney distal tubules and collecting ducts.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human NFkB p65 when phosphorylated at Ser536. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding serine 536 of human NFkB p65 protein.
