84-1128-46　ROC1 (Regulator of Cullins 1, Protein ZYP, RING Box 1, RING Box Protein 1, Ringbox Protein 1, Rbx1, RING Finger Protein 75, RNF75, ZYP Protein) 100ul　R2997-80F

特徴

• RING-box protein 1 (RBX1 or ROC1) is an essential component of two distinct but structurally related E3 ubiquitin ligase complexes, the SCF complex and the CBC(VHL) complex (1).
• RBX1 mediates the neddylation of CUL1, which activates SCF E3 ligase by facilitating the ubiquitin transfer from E2 to substrates (2-4).
• The RING finger domain of RBX1 is required for ubiquitin ligation (5).
• Two evolutionarily conserved mammalian RBX family members, RBX1/ROC1 and RBX2/ROC2/SAG, have been identified (5).
• RBX1 is constitutively expressed and binds to Cul2/VHL, while stress inducible RBX2 binds to Cul5/SOCS (6).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.