Rab10 is a member of the Ras superfamily of small Rab GTPases (1) that interacts with Msst4, myosin V (Va, Vb and Vc), and GDI as it helps mediate sorting among cellular endosomes (2-4).
Mutation analysis and GFP-fusion protein expression of Rab10 in MDCK cells determined that Rab10 plays a regulatory role in membrane protein transport between early endosome and basolateral compartments (5,6).
Rab10 associates with the GLUT4 complex as a target for AS160 and is required for translocation of GLUT4 under insulin stimulation in adipocytes (7,8).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recongnizes endogenous levels of total human Rab10 protein. Species crossreactivity: mouse and monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic peptide corresponding to residues surrounding Asp114 of human Rab10 protein. Species Sequence Homology: rat (100%)
