84-1128-36 Paip2A (PABP Binding Protein 2A) 100ul P4362-50M
特徴
- The eukaryotic mRNA 3’ poly(A) tail interacts with the 5’ cap structure to regulate translation of mRNA transcripts (1).
- The eIF4G translation initiation factor associates with poly(A) binding protein (PABP) to circularize mRNA (1,2).
- Paip1 is a PABP interacting protein that binds eIF4A to stimulate translation (3).
- A second PABP binding protein, Paip2, inhibits translation by interfering with PABP function.
- Specifically, Paip2 reduces the affinity of PABP for poly(A) RNA and competes with Paip1 for PABP binding (1,2).
- Applications:Suitable for use in Western Blot.
- Other applications not tested.
- Recommended Dilution:Western Blot: 1:1000.
- Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
- Optimal dilutions to be determined by the researcher.
- Storage and Stability:May be stored at 4°C for short-term only.
- For long-term storage, aliquot and store at -20°C.
- Aliquots are stable for at least 12 months at -20°C.
- For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Further dilutions can be made in assay buffer.
仕様
- Size：100ul
- Host：rabbit
- Source Antibody：human
- Grade：Affinity Purified
- Purity：Purified by Protein A and peptide affinity chromatography.
- Form：Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
- Specificity：Recognizes human Paip2A. Species crossreactivity: mouse, rat, monkey.
- Isotype：IgG
- Calc Applications Abbrev：WB
- Calc Crossreactivity：Hu Mk Mo Rt
- Immunogen：Synthetic peptide corresponding to human Paip2A.
- Shelf Life：1year
- Swiss Prot Number：Q9BPZ3
- EU Commodity Code：30021010
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