84-1128-34　Plastin L (PLSL, L Plastin, L-Plastin, LC64P, Lymphocyte Cytosolic Protein 1, LCP1, LCP-1, Plastin 2, Plastin-2) 100ul　P4268-01B

特徴

• Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling.
• Three plastins exhibiting differential expression are found in mam-mals and include L-plastin, T-plastin and I-plastin.
• T-plastin (plastin-3) is found in cells of most solid tissues while I- plastin (plastin-1) is expressed specifically in kidney, colon and small intestine.
• L-plastin (plastin-2) or LCP1 (lymphocyte cytosolic protein 1) is mainly expressed in hematopoietic cells and non-hematopoietic tumors, including increased expression correlated with metastatic progression in colon cancer cell lines.
• Overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of E-cadherin expression, which is characteristic of metastatic cancer cell lines.
• LCP1 becomes phosphorylated at Ser5 upon stimulation through the T-cell receptor/co-receptor complex (TCR/CD3) in association with the CD2 cell adhe-sion molecule or the CD28 receptor.
• Phosphorylation at Ser5 increases LCP1 ability to bind F-actin and increases cell motility.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.