Msh homeobox 1 (Msx1) is a Muscle Seg-ment Homeobox (Msh) gene family member that acts as a transcriptional repressor during embryonic development, playing an important role in limb pattern formation, cranio-facial development, and tooth development (1-3).
Msx1 is expressed in the mesenchyme of the developing nail bed (2) and in fetal hair follicles, epidermis and fibroblasts; reduced expression is seen in adult epithelial-derived tissues (4).
Msx1 acts in concert with the Wnt1 network to establish the midbrain dopaminergic progenator domain, a region that gives rise to neurons that are critical for normal brain function and are the cells affected in Parkinson disease (5).
Mutation in the corresponding Msx1 gene correlates with abnormal tooth development in patients diagnosed with Wolf-Hirschhorn syndrome (6).
Other genetic changes in the Msx1 gene result in Witkop Syndrome (“tooth and nail syndrome”) and cases of abnormal tooth development as-sociated with non-syndromic orofacial clefting (2,7).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recongnizes endogenous levels of human total Msx1 protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acid sequence surrounding Gly116 of human Msx1. Species sequence homology: monkey (100%)
