Mindbomb homolog 1 (MIB1) is an E3 ligase that facilitates the ubiquitination and the subsequent endocytosis of the Notch ligands, Delta and Jagged (1,2).
MIB1 appears to promote the ubiquitination and degradation of death-associated protein kinase (DAPK1) in vitro (3).
Expression of MIB1 is seen in both adult and embryonic murine tissues (4).
Recently, MIB1 was reported to regulate the extrinsic cell death pathway by binding to cellular FLICE-like inhibitory proteins (cFLIP-L and cFLIP-S), which reduces the interaction of caspase-8 with cFLIP and leads to cell death (5).
MIB1 is also involved in T and marginal zone B (MZB) cell development in the lymphopoietic niches (6).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000., incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human MIB1. Does not recognize the Ki-67 (MK167) protein.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues near the N-terminus of human MIB1.
