Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2).
IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4).
Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4+ T cells (5).
The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Ralpha, IL-2RBeta, and the common gamma chain, gammac (1,2).
IL-2Ralpha binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2RB and gammac subunits.
IL-15 also binds to IL-2Rbeta (1,2).
Gammac is used by other cytokines including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2).
Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes transfected levels of total human IL-2RB.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues surrounding Thr420 of human IL-2RB.
