B-catenin is a key downstream effector in the Wnt signaling pathway (1).
It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3).
CK1 phosphorylates B-catenin on Ser45.
This phosphorylation event primes B-catenin for subsequent phosphorylation by GSK-3 (4-6).
GSK-3B destabilizes B-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7).
Mutations in these phosphorylation sites, which result in the stabilization of B-catenin protein levels, have been found in many tumor cell lines (8).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of beta-catenin when residues Ser33, Ser37 and Thr41 are not phosphorylated. Species crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Ser37 of human beta-catenin. Species Sequence Homology: chicken, xenopus, zebrafish.
