84-1127-98　［取扱停止］LOX1 (LOX-1, hLOX-1, C-type Lectin Domain Family 8 Member A, CLEC8A, Lectin-like Oxidized LDL Receptor 1, Lectin-like oxLDL Receptor 1, Lectin-type Oxidized LDL Receptor 1, LOXIN, Oxidized Low-density Lipoprotein Receptor 1, Ox-LDL Receptor 1, OLR1, SCARE1, SLOX1) 50ug　L3514-54

特徴

• Type II membrane glycoprotein LOX-1 (lectin-like oxidized LDL receptor-1) is a 40-50kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells.
• LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions.
• Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB.
• Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1.
• Applications:Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Westen Blot.
• Other applications not tested.
• Recommended Dilutions:Flow Cytometry: 1:10; 10ul labels 10e6 cells in 100ul
• Immunoprecipitation: NeatWestern Blot: 1:10-1:100.
• Detects intact 50kD LOX-1 and also a prominent 32kD proteolytic fragmant
• Immunohistochemistry: Frozen sectionsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.