Inositol-requiring kinase 1 precursor (IRE1p) is a 108kD serine-threonine kinase/endoribonuclease also known as ERN1.
It is an endoplasmic reticulum (ER) transmembrane protein, normally bound to the ER molecular chaperones GRP78 and GRP94.
At times of ER stress, due to accumulation of unfolded proteins, IRE1p becomes dissociated from the chaperones, which allows it to activate the un-folded protein transcriptional response (UPR).
In the mammalian genome, there are two homologs Ire1alpha and Ire1beta.
Ire1alpha is expressed in all cells and tissues, Ire1beta expression is primarily restricted to intestinal epithelial cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/ml, detects a band of ~110kD in A-20 cell lysate
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human IRE1p. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A 16 amino acid peptide near the C-terminus of human IRE1p.
