There are at least three distinct chicken interferon proteins, designated as chIFN-alpha, chIFN-beta and chIFN-gamma.
chIFN-gamma was first identified when supernatant from chicken spleen cells stimulated with concanavalin A (ConA ) displayed macrophage-activating factor (MAF) activity - the principle function of mammalian type II IFN-gamma (Vonbulow et al.
1984).
Cloning of IFN-gamma cDNA from activated chicken T cells indicates that recombinant chicken IFN-gamma activates macrophages, strongly upregulating the expression of MHC class II and iNOS genes (Weining et al.
1996).
I7661-68M can be used to neutralize chIFN-gamma activity and to discriminate the antiviral activity of chIFN-gamma from that of chIFN-alpha and chIFN-beta (Weining et al.
1996).
Applications:Suitable for use in ELISA, Functional Assays, and Western Blot.
Other applications not tested.
Recommended Dilution:Neutralization: 10-50ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：chicken
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS. No preservative added (Azide free).
Specificity：Recognizes chicken interferon gamma.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Ch
Immunogen：Recombinant protein corresponding to chicken Interferon gamma.
