Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (Indirect): 1:1000-1:30,000ELISA (Coating): 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:goat
Grade:Affinity Purified
Purity:Purified by affinity chromatography from whole serum.
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:Recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. May not detect all N-terminal (NT) histidine tagged proteins.