Heme oxygenase-1 (HO-1), a 288 amino acid enzyme with a crucial role in heme catabolism, cleaving the heme ring to form biliverdin which is further processed to form bilirubin by a reductase enzyme.
HO-1 activity is highest in the spleen where it is involved in the processing of senescent erythrocytes (Delaby et al.
2012).
HO-1 expression can be increased during cellular stress, including heat shock (Ewing et al.
1992).
Baseline levels of expression are high in certain tissues, including spleen and liver.
Enriched enzyme preparations are recommended for experimental analysis.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, detects a band of ~32kD under reducing conditions
Immunohistochemistry (frozen): 1:50-1:1000Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control Tissue:Human spleenHuman kidneyPorcine arteries
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, 0.065% sodium azide.
Specificity：Recognizes human Heme oxygenase 1. Species Crossreactivity: bovine, mouse, rat and porcine. Does not react with canine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Po Rt
Immunogen：Synthetic peptide with the amino acid sequence DLSEALKEATKEVHC corresponding to residues 12-25 (+C-NH2 of human heme oxygenase 1.
