Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription.
PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of mega-N(G)-monomethylarginine and symmetric omega-N(G),N(G')-dimethylarginine in a variety of proteins.
Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein.
PRMT5 appears to have lower specific enzyme activity than PRMT1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16000Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form：Supplied as a liquid 0.5mg/ml Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes human PRMT5. Sequence Homology: mouse, rat, bovine, porcine, canine.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to RACREKDRDPEAQ, from the internal region of the protein sequence according to (NP_006100.2; NP_001034708.1.).
