SUMF1 activates sulfatases by converting their active site residue cysteine to formylglycine.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 0.5-2ug/ml detects ~37-40kDa band observed in Mouse Pancreas, Mouse Eye and Human Kidney lysates (calculated MW of 40.6kDa according to Human NP_877437.2 and 40.6kDa according to Mouse NP_666049.2)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes human SUMF1. Species crossreactivity: mouse. Sequence Homology: rat, bovine, canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to C-ETLNPKGPPSGKDR from the internal region of human SUMF1 (NP_877437.2).
