The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes.
RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene.
Hypermethylated RASSF1A promoter is observed in primary tumors.
Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes RASSF1. Sequence homology: mouse, rat.
Isotype：IgG
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to C-QAETEQKIKDYNGQ, from the internal region of RASSF1 (NP_062687.1).
