The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions.
The serine/threonine protein phosphatases (PP) are intimately involved in this process.
Protein phosphatase 4C may be involved in microtubule organization.
PP4 consists of a catalytic subunit, PPP4C, and a regulatory subunit, PPP4R1, and belongs to the PPP phosphatase family.
More specifically it is part of the PP-X subfamily.
PP4 is specifically associated with NF-kappa B, p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (Peptide): 1:16,000Western Blot: 0.1-0.3ug/mlImmunohistochemistry (FFPE): 2-4ug/ml Requires HIER using sodium citrate buffer pH6.0 prior to staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes human Protein Phosphatase 4 (formerly X) Catalytic Subunit. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to C-PQETRGIPSKKP, from the C-Terminus of human Protein Phosphatase 4 (formerly X) Catalytic Subunit Sequence homology: rat, canine.
