84-1127-09　PP2A-B’ (B56) (protein phosphatase 2A Activator, regulatory subunit 4, PPP2R4 Phosphotyrosyl Phosphatase Activator, PP2A, PP2A, Subunit B', PR53 Isoform, PR53, PTPA, Serine/threonine-protein Phosphatase 2A Regulatory Subunit B') 100ug　P9107-34V

特徴

• PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C).
• The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits.
• There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 .
• The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma .
• The B' (or B56) family consists of at least seven splice variants derived from five genes.
• RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene.
• Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals.
• PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism.
• PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid.
• It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:32,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.