The multisubunit NADH:ubiquinone oxidoreductase is the first enzyme complex in the electron transport chain of mitochondria.
By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction.
The IP fraction contains NDUFS1, NDUFS2, NDUFS3, NDUFS4, NDUFS5, NDUFS6, and NDUFA5.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes human NDUFS3. Sequence homology: bovine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-DTRPTVRPRNDVAHK, from the internal region of NDUFS3 human (NP_004542.1).
