Chemokine (C-X-C motif) ligand 2 (CXCL2) is a small cytokine belonging to the CXC chemokine family that is also called macrophage inflammatory protein 2-alpha (MIP2-alpha), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2).
CXCL2 is 90% identical in amino acid sequence as a related chemokine, CXCL1.
This chemokine is secreted by monocytes and macrophages and is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells.
The gene for CXCL2 is located on human chromosome 4 in a cluster of other CXC chemokines.[CXCL2 mobilizes cells by interacting with a cell surface chemokine receptor called CXCR2.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.2-0.6ug/ml detects ~17Da band observed in Human Duodenum lysates (calculated MW of 11.4kDa according to NP_002080.1)
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes human CXCL2/MIP-2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-KKIIEKMLKNGKSN from the C-Terminus of human CXCL2/MIP-2 (NP_002080.1).
