IL-12 is a heterodimer composed of subunits IL-12A p35 and IL-12B p40.
Autocrine IL-12 is responsible for the differentiation of naive CD4+T cells into type 1 helper T cells (Th1) that produce interferon-_ (IFN-_).
Expression of STAT 4, a signal transduction molecule important in cytokine-induced gene expression, appears central to IL-12 induced Th1 production of IFN-_.
It also activates production of tumor necrosis factor (TNF) from T and natural killer cells.
IL-12 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes mouse Il12b/Il12p40. Species crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to C-EARQQNKYENYS from the internal region of mouse Il12b/Il12p40 (NP_032378.1).
