Met-enkephalin (tyr-gly-gly-phe-met) and leu-enkephalin (tyr-gly-gly-phe-leu) are pentapeptides which compete with and mimic the effects of opiate drugs.
Although interest in enkephalins stems largely from their possible role in the brain, the richest source of these peptides is the adrenal gland.
The amino acid sequence shows that the precursor is 267 amino acids long and contains 6 interspersed Met-enkephalin sequences and 1 Leu-enkephalin sequence.
The precursor does not contain the sequences of dynorphin, alpha-neo-endorphin or beta-endorphin.
(Because of structural similarities it had been postulated that beta-endorphin is precursor of Met-enkephalin, and that dynorphin or alpha-neo-endorphin is precursor of Leu-enkephalin.)
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes PENK. Sequence homology: mouse, rat.
Isotype：IgG
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to C-YKDSSKQDESH, from the internal region of PENK (NP_001002927.1).
