Eukaryotic DNA ligases are ATP-dependent enzymes that catalyze the joining of single and double stranded DNA breaks, which is an essential step in DNA repair, replication and recombination.
There are at least 4 DNA ligases, termed DNA ligase I-IV, have been cloned and characterized.
DNA ligase-I is functionally homologous to the DNA ligase encoded by the S.
cervisiae CDC9 gene.
The joining of Okazaki fragments during lagging strand DNA replication is due to DNA ligase-I.
DNA ligase-I (919 aa, chromosome 19q13.2-q13.3; mouse 916 aa, rat 919 aa) is a nuclear protein.
Its deficiency causes immunodeficiency and cellular hypersensitivity of DNA-damaging agents.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes human LIG1/DNA Ligase 1. Sequence homology: rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-RVREDKQPEQATTS, from the internal region (near C-Terminus) of human LIG1/DNA Ligase I (NP_000225.1).
