CHST5 (Carbohydrate sulfotransferase 5; also GlcNAc6ST-3, I-GlcNAc6ST and GST4 alpha) is likely a 53-56kD member of the sulfotransfease family of enzymes.
It is a Golgi-embedded enzyme that is found in T cells, B cells and intestinal epithelium.
It appears to act only on short carbohydrates and transfers sulfate to position #6 of mucin-associated GlcNAc.
Human CHST5 is a 390aa type II transmembrane protein.
It contains a short cytoplasmic segment (aa1-9) and a long luminal domain (aa28-390) that contains an enzymatic region (aa180-358).
There is one alternate start site that is 22aa upstream of the standard site.
Humans have a GST4-alpha and -beta gene, the result of gene duplication.
Over aa28-390, these two gene products share 87% aa identity.
Mouse has only one GST4 gene, and it is functionally related to human GST4- beta.
Over aa 28-390, human GST4-alpha/CHST5 is 78% aa identical to mouse GST4.
Applications:Suitable for use in Peptide ELISA.
Other applications not tested.
Recommended Dilutions:Peptide ELISA: 1:32,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes Carbohydrate Sulfotransferase 5. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to C-DSFKWASSTEKQPE from the C Terminus of mouse Carbohydrate Sulfotransferase 5 (NP_064334.1).
