CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD).
The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene.
CAMKIIA is required for hippocampal long-term potentiation (LTP) and spatial learning.
In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity.
Two transcript variants encoding distinct isoforms have been identified for this gene.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
~55-65kD bands observed in Human Brain (Cerebellum) and Mouse Brain lysates (MW 55.3kD and 54.1)Immunohistochemistry(Paraffin): 3-6ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity：Recognizes human Camk2a. Species crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to C-PRTAQSEETRVWHR, from the internal region of human Camk2a. Species Sequence homology: rat and canine.
