The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels.
This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors.
Only the fully processed form can bind and activate VEGFR-2 receptors.
This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D).
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml K562 lysates under reducing conditions.
Immunohistochemistry: 5-15ug/ml Immersion fixed paraffin embedded sections of human colon cancer tissueOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:Recognizes human Vascular Endothelial Growth Factor C. Species Crossreactivity: <1% with rhVEGF-D and rhVEGF-A.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human VEGF C, aa104-330 expressed in E. coli.