Midkine (MK, also called neurite growth promoting factor 2, NEGF-2), a product of a retinoic acid responsive gene, is a secreted 13 kD protein belonging to the family of heparin binding growth/differen-tiation factors.
MK shares 45% sequence identity with other member of this family called Pleiotrophin (HB-GAM).
Midkine is composed of two domains held together by disulfide linkages.
The C-terminally located domain contains two heparin binding sites and is usually responsible for midkine activity.
Part of the MK activity is enhanced by dimerization of MK.
Midkine has been found in vertebrates from human to zebrafish and is most strongly expresed in midgestation.
In the adult MK expression is restricted.
In addition to normal development, MK is also involved in the pathogenesis of diseases e.g.
inflammatory diseases, human carcinomas such as esophageal, stomach, colon, pancreatic, thyroid, lung, urinary, hepatocellular, neuroblastoma, glioblastoma, Wilm´s tumor etc.
High MK levels are associated with poor prognosis in some type of cancer.
The increased expresion in many carcinomas indicates that MK can be applied to the diagnosis of malignancy.
Midkine is expressed during the reparative stage of bone fractures, also supresses infection of certain viruses including HIV in target cells.
Anti-apoptotic and cell protecting activity of midkine makes it to be a promissing in therapy.
Application:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage ad Stabillty:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2. No preservative added. Reconstitute with 50ul sterile dH2O. Let the lyophilized pellet dissolve completely.
Specificity：Recognizes human Midkine.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant human Midkine expressed in E. coli
