Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kD forming a heterotrimeric protein complex with beta and gamma subunits.
Two isoforms of the alpha subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.
This antibody has been used successfully for the detection of G(o) in Drosophila by Western Blot (Guo et al.
2011).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:500-1:1000Western Blot: detects a band at ~39kD when used under reducing conditions against rat brain
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes mouse G Protein alpha 0. Species Crossreactivity: drosophila
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Dr Mo
Immunogen：Synthetic peptide corresponding to the aa345-354 (C-ANNLRGCGLY) of the native isoform 1 molecule conjugated to KLH.
