Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone.
It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD.
The protein has been found in osteoblasts and osteocytes.
BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells.
Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers.
Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling.
Applications:Suitable for use in ELISA, Western Blot, RIA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry (Paraffn): 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：20ul
Host：chicken
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder in PBS, pH 7.4. Reconstitute in ddH2O.
Specificity：Recognizes human Bone Sialoprotein (native protein). There were no cross reactivities obtained with human Osteonectin or human Osteopontin.
