Osteopontin is a single-chain polypeptide with a molecular weight of ~35kD.
It is a glycoprotein first identified in osteoblasts.
It is biosynthesized by a variety of tissue types including preosteoblasts, osteoblasts, osteocytes, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, macrophages, smooth muscle, and endothelial cells.
Osteopontin is overexpressed in a variety of cancers, including lung cancer, breast cancer, colorectal cancer, stomach cancer, ovarian cancer, melanoma and mesothelioma.
It may contribute to kidney stone formation and both glomerulonephritis and tubulointerstitial nephritis and is also found in atheromatous plaques within arteries.
Research has implicated osteopontin in excessive scar-forming and a gel has been developed to inhibit its effect.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1000Immunohistochemistry (Paraffin): 1:1:100-1:1500Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：20ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with sterile ddH2O.
Specificity：Recognizes human Osteopontin (aa59-74). Species Crossreactivity: mouse. No crossreactivity with human Osteonectin, and human Bone Sialoprotein.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa59-74, PSKSNESHDHMDDMDD from human Osteopontin, conjugated to KLH.
