SPARC, also known as Osteonectin and basement membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains.
It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta.
SPARC may be involved in cell proliferation, repair of tissue damage and morphogenetic processes such as modeling of extracellular matrix.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:3000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 20ul or 100ul sterile ddH2O.
Specificity：Recognizes synthetic human Osteonectin (aa272-286), and recombinant human Osteonectin. Does not crossreact with human Osteopontin or human Bone Sialoprotein.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa272-286, CFGIKQKDIDKDLVI, from human Osteonectin (GeneID 6678, Swiss-Prot P09486).
